ABSTRACT
<p><b>OBJECTIVE</b>To study action of Cryptoporus volvatus ferment substance (CVFS) on leukotriene production of polymorphonuclear leukocytes in rats.</p><p><b>METHODS</b>The level of slow reaction substance (SRS) and leukotriene B4 (LTB4) in polymorphonuclear leukocytes (PMNs) in rats in vitro were determined with bioassay and HPLC.</p><p><b>RESULTS</b>CVFS 0.9, 2.7 g.kg-1 by ig significantly inhibited SRS and LTB4 production in PMNs in rats in vivo.</p><p><b>CONCLUSION</b>The inhibition effect of CVFS on SRS and LTB4 release may be related to its mechanism of anti-inflammation and anti-asthma.</p>
Subject(s)
Animals , Female , Male , Rats , Anti-Asthmatic Agents , Pharmacology , Anti-Inflammatory Agents, Non-Steroidal , Pharmacology , Cell Separation , Dose-Response Relationship, Drug , Drugs, Chinese Herbal , Pharmacology , Guinea Pigs , Leukotriene B4 , Metabolism , Neutrophils , Metabolism , Polyporaceae , Chemistry , Rats, Sprague-Dawley , SRS-A , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To study inhibitory the effects of Cryptoporus volvatus ferment substance(CVFS) on leukotriene production in vitro from neutrophils in rats.</p><p><b>METHODS</b>Neutrophil aggregation was induced by intraperitoneal injection of glycogen in rats. After 16 h, intraperitoneal lavage fluid(PLF) was collected and neutrophils were removed. Neutrophils were stimulated by calcium ionophore A23187 in vitro to produce leukotriene B(4), C(4), D(4). The concentrations of leukotriene B(4), C(4) and D(4) were measured by reversed-phase high-performance liquid chromatography(HPLC).</p><p><b>RESULT</b>CVFS at 0.25, 1, 4 mg x L(-1)decreased leukotriene B(4), C(4), D(4) release from neutrophils in a concentration-dependent manner. Inhibitory rate of CVFS 0.25, 1, 4 mg x L(-1 )on A23187-induced leukotriene B(4) production was 27.4%, 54.2% and 78.8%(P<0.05), respectively. Inhibitory rate of leukotriene C(4) production was 65.1%, 74.3 and 79.0%(P<0.05), respectively. Inhibitory rate of leukotriene D(4) production was 55.6%, 60.9% and 72.8%(P<0.05), respectively.</p><p><b>CONCLUSION</b>The results suggest that suppression of leukotriene release may be a mechanism of the anti-inflammation and anti-asthma effects of CVFS.</p>